Gender assessment of the gut microbiome in obese patients

The aim . To assess the relationship between body mass index (BMI) and gut bacteria in men women with obesity. Materials methods study included 56 overweight patients, divided into 2 groups. first group consisted of 27 (the average age was 62 ± 2.2 years), second — 29 55 9 years). Quetelet (kg / m ) calculated for all patients. microbiome, method polymerase chain reaction real time (RT-PCR) metagenomic sequencing were used. DNA from feces isolated using Express-DNA-Bio isolation kit (AlkorBio, Russia). carry out RT-PCR, a set reagents “Colonoflor-16” (“Alfalab”, Russia) For microbiome sequencing, libraries prepared Illumina Nextera Sample Preparation Kit primers corresponding to V3 V4 regions 16S rRNA gene. fecal samples carried gene on platform (MiSeq sequencer). Results It revealed that higher total number bacteria, an increased content Bacteroides fragilis Faeca-libacterium prausnitzii, is statistically significantly more common than men. Strong negative correlations found BMI bacterial mass, among grade I In men, correlation Faecalibacterium prausnitzii ratio. Conclusions patients have significant associations BMI, probably can affect formation metabolic disorders greater extent clarify identified trends patterns this pilot study, further large additional analyzes metagenome (16S rRNA) metabolome, transcriptome, allowing control expression key enzymes, largely associated compositional features microbiocenosis, required.